User:Michael F. Nagle/Notebook/Chem 571/2012/09/12: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(6 intermediate revisions by the same user not shown)
Line 7: Line 7:
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Objectives==
==Objectives==
*With UV/Vis, see how varying concentrations of Tris at pH=10 affect dissolution of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] fibers
*With UV/Vis, see how varyious concentrations of Tris at pH=10 dissolve [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] fibers
*Prepare solution of HAuCl<sub>4</sub> and BSA at a concentration that allows for dissolution rather than precipitation of fibers. The particles in solution will be mixed with Tris buffer to see how they are affected.
*Prepare solution of HAuCl<sub>4</sub> and BSA at a concentration that will have AuNPs in solution rather than fibers. The particles in solution will be mixed with Tris buffer to see how they are affected.
==Procedure==
==Procedure==
#Measure absorbancy of gold nanoparticles in solutions with varying concentrations of Tris buffer
* Solutions with a 170 mole ratio of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/11|yesterday]] were centrifuged at 3000rpm and 10<sup>o</sup>C for 5 minutes.
## Solutions with a 170 mole ratio of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] were put in a centrifuge at 3000rpm and 10<sup>o</sup>C for 5 minutes.
** Water was removed with a pipette
### Water was removed with a pipette
** A serial dilution with Tris was completed exactly as on [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/04|9/4]], with Tris stock at pH 10 made that day.
## Solutions of HAuCl<sub>4</sub> and BSA with Tris were put in UV/Vis again to obtain a third set of spectra, to be compared to the spectra from [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/05|the day before]]
** Solutions were analyzed via UV/Vis
## Solutions were made with a mole ratio (HAuCl<sub>4</sub> to BSA)of 70.
* A 70 [Au/BSA] solution was prepared just like the one on [[User:Michael_F._Nagle/Notebook/Chem_571/2012/08/29|8/29]], using the same stock solutions. These went in oven at 85<sup>o</sup>C for 4 hours.
##2.4268M BSA solution was used
###m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub>
### 24.266μM * (volume) = 1μM * 6mL
### .247mL BSA solution
## 2150μM HAuCl4 solution was used
### 2150μM * (volume) = 6mL *(1μM*70)
### .195mL HAuCl<sub><sub>4</sub></sub> solution
## 6-.195mL BSA -.247mL HAuCl4 = 5.558mL H<sub>2</sub>O was added
#Solutions went in oven at 85<sup>o</sup>C from 2:32-6:32


==Data==
==Data==
[[Image:AuNP_in_tris_buffer_pH_10.xlsx]]
[[Image:Untitled.bmp]]


==Discussion==
==Discussion==
*The three sets of spectra for Tris show almost no change in absorbancy. There is a drop in absorbancy for 1*10<sup>-4</sup>M Tris solution, but not in any others. More trials should be run to determine if this was due to noise or a change in dissolution.
*Just like the [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/11|sets with Tris at pH 8]], there was no change in absorbance at 528nm relative to background. The entire background dropped over the course of the first 2 sets, just like it did between the 2nd and 3rd in the trials at pH 8
 
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 14:44, 17 September 2012 (EDT)''':see previous entries for notes on embeding graphs and describing calculations. more details needed in general. these are not trials you ran but time points. how did the solution change as a function of time. at 1 hour after adding tris versus after 24 hours or after a week?
<!-- ##### DO NOT edit below this line unless you know what you are doing. ##### -->
|}
|}


__NOTOC__
__NOTOC__

Revision as of 21:17, 6 December 2012

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  • With UV/Vis, see how varyious concentrations of Tris at pH=10 dissolve HAuCl4 and BSA fibers
  • Prepare solution of HAuCl4 and BSA at a concentration that will have AuNPs in solution rather than fibers. The particles in solution will be mixed with Tris buffer to see how they are affected.

Procedure

  • Solutions with a 170 mole ratio of HAuCl4 and BSA prepared yesterday were centrifuged at 3000rpm and 10oC for 5 minutes.
    • Water was removed with a pipette
    • A serial dilution with Tris was completed exactly as on 9/4, with Tris stock at pH 10 made that day.
    • Solutions were analyzed via UV/Vis
  • A 70 [Au/BSA] solution was prepared just like the one on 8/29, using the same stock solutions. These went in oven at 85oC for 4 hours.

Data

Discussion

  • Just like the sets with Tris at pH 8, there was no change in absorbance at 528nm relative to background. The entire background dropped over the course of the first 2 sets, just like it did between the 2nd and 3rd in the trials at pH 8


Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Michael_F._Nagle/Notebook/Chem_571/2012/09/12&oldid=662696"