User:Michael F. Nagle/Notebook/Chem 571/2012/09/12: Difference between revisions

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==Objectives==
==Objectives==
*With UV/Vis, see how varying concentrations of Tris at pH=10 affect dissolution of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] fibers
*With UV/Vis, see how varyious concentrations of Tris at pH=10 dissolve [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] fibers
*Prepare solution of HAuCl<sub>4</sub> and BSA at a concentration that allows for dissolution rather than precipitation of fibers. The particles in solution will be mixed with Tris buffer to see how they are affected.
*Prepare solution of HAuCl<sub>4</sub> and BSA at a concentration that will have AuNPs in solution rather than fibers. The particles in solution will be mixed with Tris buffer to see how they are affected.
==Procedure==
==Procedure==
## Solutions with a 170 mole ratio of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] were put in a centrifuge at 3000rpm and 10<sup>o</sup>C for 5 minutes.
* Solutions with a 170 mole ratio of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/11|yesterday]] were centrifuged at 3000rpm and 10<sup>o</sup>C for 5 minutes.
### Water was removed with a pipette
** Water was removed with a pipette
## Solutions of HAuCl<sub>4</sub> and BSA with Tris were put in UV/Vis again to obtain a third set of spectra, to be compared to the spectra from [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/05|the day before]]
** A serial dilution with Tris was completed exactly as on [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/04|9/4]], with Tris stock at pH 10 made that day.
## Solutions were made with a mole ratio (HAuCl<sub>4</sub> to BSA)of 70.
** Solutions were analyzed via UV/Vis
##2.4268M BSA solution was used
* A 70 [Au/BSA] solution was prepared just like the one on [[User:Michael_F._Nagle/Notebook/Chem_571/2012/08/29|8/29]], using the same stock solutions. These went in oven at 85<sup>o</sup>C for 4 hours.
###m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub>
### 24.266μM * (volume) = 1μM * 6mL
### .247mL BSA solution
## 2150μM HAuCl4 solution was used
### 2150μM * (volume) = 6mL *(1μM*70)
### .195mL HAuCl<sub><sub>4</sub></sub> solution
## 6-.195mL BSA -.247mL HAuCl4 = 5.558mL H<sub>2</sub>O was added
##Solutions went in oven at 85<sup>o</sup>C from 2:32-6:32


==Data==
==Data==
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==Discussion==
==Discussion==
*The three sets of spectra for Tris show almost no change in absorbancy. There is a drop in absorbancy for 1*10<sup>-4</sup>M Tris solution, but not in any others. More trials should be run to determine if this was due to noise or a change in dissolution over time.
*Just like the [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/11|sets with Tris at pH 8]], there was no change in absorbance at 528nm relative to background. The entire background dropped over the course of the first 2 sets, just like it did between the 2nd and 3rd in the trials at pH 8
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Revision as of 21:17, 6 December 2012

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Objectives

  • With UV/Vis, see how varyious concentrations of Tris at pH=10 dissolve HAuCl4 and BSA fibers
  • Prepare solution of HAuCl4 and BSA at a concentration that will have AuNPs in solution rather than fibers. The particles in solution will be mixed with Tris buffer to see how they are affected.

Procedure

  • Solutions with a 170 mole ratio of HAuCl4 and BSA prepared yesterday were centrifuged at 3000rpm and 10oC for 5 minutes.
    • Water was removed with a pipette
    • A serial dilution with Tris was completed exactly as on 9/4, with Tris stock at pH 10 made that day.
    • Solutions were analyzed via UV/Vis
  • A 70 [Au/BSA] solution was prepared just like the one on 8/29, using the same stock solutions. These went in oven at 85oC for 4 hours.

Data

Discussion

  • Just like the sets with Tris at pH 8, there was no change in absorbance at 528nm relative to background. The entire background dropped over the course of the first 2 sets, just like it did between the 2nd and 3rd in the trials at pH 8


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