User:Michael F. Nagle/Notebook/Chem 571/2012/09/12: Difference between revisions
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==Entry title== | ==Entry title== | ||
#Measure absorbancy of gold nanoparticles in solutions with varying concentrations of Tris buffer | #Measure absorbancy of gold nanoparticles in solutions with varying concentrations of Tris buffer | ||
## 1mol Tris /1L = 1M | ## Solutions with a 170 mole ratio of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] were put in a centrifuge at 3000rpm and 10<sup>o</sup>C for 5 minutes. | ||
##121.14g/mol Tris | ### Water was removed with a pipette | ||
## 12.114g Tris was put in 100mL to make 1M stock solution | ## Tris stock was prepared | ||
### 1mol Tris /1L = 1M | |||
###121.14g/mol Tris | |||
### 12.114g Tris was put in 100mL to make 1M stock solution | |||
### 9mL water was added to each tube and a serial dilution was done with Tris. | |||
#Solution was made with a mole ratio of 70 | #Solution was made with a mole ratio of 70 | ||
##2.4268M BSA solution was used | ##2.4268M BSA solution was used |
Revision as of 12:21, 12 September 2012
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