User:Michael F. Nagle/Notebook/Chem 571/2012/09/12: Difference between revisions

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###121.14g/mol Tris  
###121.14g/mol Tris  
### 12.114g Tris was put in 100mL to make 1M stock solution
### 12.114g Tris was put in 100mL to make 1M stock solution
### 9mL water was added to each tube and a serial dilution was done with Tris.
### 9mL water was added to each tube and a serial dilution was done with Tris in 8 tubes.
## Absorbance was measured in each of the 8 tubes 3 times using UV/Vis
#Solution was made with a mole ratio of 70
#Solution was made with a mole ratio of 70
##2.4268M BSA solution was used
##2.4268M BSA solution was used

Revision as of 12:23, 12 September 2012

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  1. Measure absorbancy of gold nanoparticles in solutions with varying concentrations of Tris buffer
    1. Solutions with a 170 mole ratio of HAuCl4 and BSA were put in a centrifuge at 3000rpm and 10oC for 5 minutes.
      1. Water was removed with a pipette
    2. Tris stock was prepared
      1. 1mol Tris /1L = 1M
      2. 121.14g/mol Tris
      3. 12.114g Tris was put in 100mL to make 1M stock solution
      4. 9mL water was added to each tube and a serial dilution was done with Tris in 8 tubes.
    3. Absorbance was measured in each of the 8 tubes 3 times using UV/Vis
  2. Solution was made with a mole ratio of 70
    1. 2.4268M BSA solution was used
      1. m1v1=m2v2
      2. 24.266μM * (volume) = 1μM * 6mL
      3. .247mL BSA solution
    2. 2150μM HAuCl4 solution was used
      1. 2150μM * (volume) = 6mL *(1μM*70)
      2. .195mL HAuCl4 solution
    3. 6-.195mL BSA -.247mL HAuCl4 = 5.558mL H2O was added
  3. Solutions went in oven at 85oC from 2:32-6:32



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