User:Michael F. Nagle/Notebook/Chem 571/2012/09/12: Difference between revisions

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*Prepare solution of HAuCl<sub>4</sub> and BSA at a concentration that allows for dissolution rather than precipitation of fibers. The particles in solution will be mixed with Tris buffer to see how they are affected.
*Prepare solution of HAuCl<sub>4</sub> and BSA at a concentration that allows for dissolution rather than precipitation of fibers. The particles in solution will be mixed with Tris buffer to see how they are affected.
==Procedure==
==Procedure==
#Measure absorbancy of gold nanoparticles in solutions with varying concentrations of Tris buffer
## Solutions with a 170 mole ratio of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] were put in a centrifuge at 3000rpm and 10<sup>o</sup>C for 5 minutes.
## Solutions with a 170 mole ratio of [[AU_Biomaterials_Design_Lab:Materials/HAuCl4|HAuCl<sub>4</sub>]] and [[AU_Biomaterials_Design_Lab:Materials/BSA|BSA]] were put in a centrifuge at 3000rpm and 10<sup>o</sup>C for 5 minutes.
### Water was removed with a pipette
### Water was removed with a pipette

Revision as of 11:26, 25 September 2012

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Objectives

  • With UV/Vis, see how varying concentrations of Tris at pH=10 affect dissolution of HAuCl4 and BSA fibers
  • Prepare solution of HAuCl4 and BSA at a concentration that allows for dissolution rather than precipitation of fibers. The particles in solution will be mixed with Tris buffer to see how they are affected.

Procedure

    1. Solutions with a 170 mole ratio of HAuCl4 and BSA were put in a centrifuge at 3000rpm and 10oC for 5 minutes.
      1. Water was removed with a pipette
    2. Solutions of HAuCl4 and BSA with Tris were put in UV/Vis again to obtain a third set of spectra, to be compared to the spectra from the day before
    3. Solutions were made with a mole ratio (HAuCl4 to BSA)of 70.
    4. 2.4268M BSA solution was used
      1. m1v1=m2v2
      2. 24.266μM * (volume) = 1μM * 6mL
      3. .247mL BSA solution
    5. 2150μM HAuCl4 solution was used
      1. 2150μM * (volume) = 6mL *(1μM*70)
      2. .195mL HAuCl4 solution
    6. 6-.195mL BSA -.247mL HAuCl4 = 5.558mL H2O was added
    7. Solutions went in oven at 85oC from 2:32-6:32

Data

File:AuNP in tris buffer pH 10.xlsx

Discussion

  • The three sets of spectra for Tris show almost no change in absorbancy. There is a drop in absorbancy for 1*10-4M Tris solution, but not in any others. More trials should be run to determine if this was due to noise or a change in dissolution.
  • Abigail E. Miller 14:44, 17 September 2012 (EDT):see previous entries for notes on embeding graphs and describing calculations. more details needed in general. these are not trials you ran but time points. how did the solution change as a function of time. at 1 hour after adding tris versus after 24 hours or after a week?


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