User:Michael F. Nagle/Notebook/Chem 571/2012/09/18

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(Autocreate 2012/09/18 Entry for User:Michael_F._Nagle/Notebook/Chem_571)
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==Entry title==
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==Objectives==
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* Insert content here...
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* Grow E. Coli in [[AU Biomaterials Design Lab:Materials/LB|LB]] broth, for the purpose of expressing ADA.
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==Procedure==
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# In four 2.8L flasks, broth was created with 25g [[AU Biomaterials Design Lab:Materials/LB|LB]] and 1L H<sub>2</sub>O. Four more smaller 250mL flasks were prepared using this protocol for making [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|Starter Culture]]
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# The starter culture was steamed in the autoclave following [[AU Biomaterials Design Lab:Protocols/Autoclave|this protocol]]

Revision as of 13:10, 18 September 2012

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Objectives

  • Grow E. Coli in LB broth, for the purpose of expressing ADA.

Procedure

  1. In four 2.8L flasks, broth was created with 25g LB and 1L H2O. Four more smaller 250mL flasks were prepared using this protocol for making Starter Culture
  2. The starter culture was steamed in the autoclave following this protocol



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