User:Michael F. Nagle/Notebook/Chem 571/2012/09/18: Difference between revisions
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##The antibiotic was allowed to warm to room temperature and then inserted to the broth. The remaining Kanamycin went back in the freezer. | ##The antibiotic was allowed to warm to room temperature and then inserted to the broth. The remaining Kanamycin went back in the freezer. | ||
#The foil caps on the broth flasks was sterilized with a bunsen burner after each time they were removed and placed back on the flasks. Metal forceps, which were also sterilized with flame between uses, were used to pick up a splint, which was used to pick up one colony of E. Coli and transfer it to the broth. A different split was used each time. | #The foil caps on the broth flasks was sterilized with a bunsen burner after each time they were removed and placed back on the flasks. Metal forceps, which were also sterilized with flame between uses, were used to pick up a splint, which was used to pick up one colony of E. Coli and transfer it to the broth. A different split was used each time. | ||
# | #E. Coli transformed with a plasmid coding for Adenosine Deaminase (ADA)was inserted into the broth. | ||
#The flasks were | #The flasks were left overnight in an orbital incubator shaker at 37°C and 237 rpm | ||
==Discussion== | ==Discussion== |
Revision as of 17:44, 7 December 2012
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