User:Michael F. Nagle/Notebook/Chem 571/2012/09/18: Difference between revisions
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==Procedure== | ==Procedure== | ||
#Starter Culture and Expression Culture | #Starter Culture and Expression Culture | ||
##[http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media | ##[http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media Starter culture media]and [http://openwetware.org/wiki/AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media expression culture media] were made following procedure. 25mL water was used instead of 35mL in making the starter culture. | ||
##The broth flasks were capped with foil. | ##The broth flasks were capped with foil. | ||
##The flasks were put in the autoclave at 121°C for 60 minutes. Due to limited space, the flasks had to be autoclaved in two batches. | ##The flasks were put in the autoclave at 121°C for 60 minutes. Due to limited space, the flasks had to be autoclaved in two batches. |
Revision as of 16:34, 1 October 2012
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Objectives
Procedure
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