User:Michael F. Nagle/Notebook/Chem 571/2012/09/18

From OpenWetWare
< User:Michael F. Nagle‎ | Notebook‎ | Chem 571‎ | 2012‎ | 09
Revision as of 11:10, 18 September 2012 by Michael F. Nagle (talk | contribs)
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objectives

  • Grow E. Coli in LB broth, for the purpose of expressing ADA.

Procedure

  1. In four 2.8L flasks, broth was created with 25g LB and 1L H2O. Four more smaller 250mL flasks were prepared using this protocol for making Starter Culture
  2. The starter culture was steamed in the autoclave following this protocol



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Michael_F._Nagle/Notebook/Chem_571/2012/09/18&oldid=627213"