User:Michael F. Nagle/Notebook/Chem 571/2012/09/19: Difference between revisions
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##.05mM*6mL=1000mM*x | ##.05mM*6mL=1000mM*x | ||
##.0003mL Tris stock for .05mM solution | ##.0003mL Tris stock for .05mM solution | ||
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 11:34, 7 October 2012 (EDT)''':why are you making these buffers? how much as added to what concentrations of HAuCl4 nad BSA? is this before or after AuNP formation? | |||
==Observations== | ==Observations== | ||
One of the flasks with broth appeared a different shade of yellow this morning prior to centrifuging. | One of the flasks with broth appeared a different shade of yellow this morning prior to centrifuging. | ||
*'''[[User:Abigail E. Miller|Abigail E. Miller]] 11:34, 7 October 2012 (EDT)''':why is this important? did this change your procedure? | |||
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Revision as of 08:34, 7 October 2012
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Objectives
Procedure
Starter cultures were centrifuged for 15 minutes at 4500rpm and resuspended in 4mL LB broth.
ObservationsOne of the flasks with broth appeared a different shade of yellow this morning prior to centrifuging.
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