User:Michael F. Nagle/Notebook/Chem 571/2012/09/19: Difference between revisions
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|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
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*Tris added to Au/BSA | *Tris added to Au/BSA | ||
**In order to determine how various concentrations of tris affect AuNPs in solution, tris was added to 70 [HAuCl<sub>4</sub>/BSA] solutions prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/12|last week]] at .05mM, .5mM, 5mM, 50mM, 100mM, 200mM, 500mM and 1M. These [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/25|are to be analyzed by UV/Vis]]. | **In order to determine how various concentrations of tris affect AuNPs in solution, tris was added to 70 [HAuCl<sub>4</sub>/BSA] solutions prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/12|last week]] at .05mM, .5mM, 5mM, 50mM, 100mM, 200mM, 500mM and 1M. These [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/25|are to be analyzed by UV/Vis]]. | ||
*** | ***1M stock solution for tris was made | ||
****1mol/L *.025L = .025mol Tris needed | ****1mol/L *.025L = .025mol Tris needed | ||
****.025mol * 121.14g/mol = 3.0285g Tris weighed | ****.025mol * 121.14g/mol = 3.0285g Tris weighed | ||
***The following calculations were completed to determine how much tris was needed in each solution for concentrations 1M, 500mM, 200mM and 100mM. A serial dilution was done to prepare concentrations .05mM-50mM from the 500mM solution. | ***The following calculations were completed to determine how much tris was needed in each solution for concentrations 1M, 500mM, 200mM and 100mM. A serial dilution was done to prepare concentrations .05mM-50mM from the 500mM solution. | ||
***m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | ****m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | ||
***1000mM*6mL=1000mM*x | ****1000mM*6mL=1000mM*x | ||
****6mL Tris stock for 1M solution | *****6mL Tris stock for 1M solution | ||
***200mM*6mL=1000mM*x | ****200mM*6mL=1000mM*x | ||
****1.2mL Tris stock for 200mM solution | *****1.2mL Tris stock for 200mM solution | ||
***100mM*6mL=1000mM*x | ****100mM*6mL=1000mM*x | ||
****.6mL Tris stock for 100mM solution | *****.6mL Tris stock for 100mM solution | ||
__NOTOC__ | __NOTOC__ |
Latest revision as of 22:01, 26 September 2017
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Objectives
Procedure
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