User:Michael F. Nagle/Notebook/Chem 571/2012/09/19: Difference between revisions
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****.025mol * 121.14g/mol = 3.0285g Tris weighed | ****.025mol * 121.14g/mol = 3.0285g Tris weighed | ||
***The following calculations were completed to determine how much tris was needed in each solution for concentrations 1M, 500mM, 200mM and 100mM. A serial dilution was done to prepare concentrations .05mM-50mM from the 500mM solution. | ***The following calculations were completed to determine how much tris was needed in each solution for concentrations 1M, 500mM, 200mM and 100mM. A serial dilution was done to prepare concentrations .05mM-50mM from the 500mM solution. | ||
***m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | ****m<sub>1</sub>v<sub>1</sub>=m<sub>2</sub>v<sub>2</sub> | ||
***1000mM*6mL=1000mM*x | ****1000mM*6mL=1000mM*x | ||
****6mL Tris stock for 1M solution | *****6mL Tris stock for 1M solution | ||
***200mM*6mL=1000mM*x | ****200mM*6mL=1000mM*x | ||
****1.2mL Tris stock for 200mM solution | *****1.2mL Tris stock for 200mM solution | ||
***100mM*6mL=1000mM*x | ****100mM*6mL=1000mM*x | ||
****.6mL Tris stock for 100mM solution | *****.6mL Tris stock for 100mM solution | ||
__NOTOC__ | __NOTOC__ |
Revision as of 21:41, 6 December 2012
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