User:Michael F. Nagle/Notebook/Chem 571/2012/09/19: Difference between revisions
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****100mM*6mL=1000mM*x | ****100mM*6mL=1000mM*x | ||
*****.6mL Tris stock for 100mM solution | *****.6mL Tris stock for 100mM solution | ||
**The contents of each tube were as follows: | |||
|+ Amount of Tris stock and water in each tube | |||
! scope="col" |'''Tris Buffer concentration''' | |||
! scope="col" |'''amount of water (mL)''' | |||
! scope="col" |'''amount of tris (mL)''' | |||
|- | |||
| .05 mM||0.9938||0.0625 | |||
|- | |||
| .5 mM||0.9875||0.0125 | |||
|- | |||
| 5 mM||0.975||0.025 | |||
|- | |||
| 50 mM||0.95||0.05 | |||
|- | |||
| 100 mM||0.9||0.1 | |||
|- | |||
| 200 mM||0.8||0.2 | |||
|- | |||
| 500 mM||0.5||0.5 | |||
|- | |||
| 1 M||0||1 | |||
|} | |||
__NOTOC__ | __NOTOC__ |
Revision as of 21:43, 6 December 2012
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Objectives
Procedure
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Tris Buffer concentration | amount of water (mL) | amount of tris (mL) |
---|---|---|
.05 mM | 0.9938 | 0.0625 |
.5 mM | 0.9875 | 0.0125 |
5 mM | 0.975 | 0.025 |
50 mM | 0.95 | 0.05 |
100 mM | 0.9 | 0.1 |
200 mM | 0.8 | 0.2 |
500 mM | 0.5 | 0.5 |
1 M | 0 | 1 |