User:Michael F. Nagle/Notebook/Chem 571/2012/09/26: Difference between revisions
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==Data== | ==Data== | ||
These absorbance peaks show the amount of ADA in each tube. | These absorbance peaks show the amount of ADA in each tube. | ||
[[ | [[Image:ADA HisTrap2.PNG]] | ||
==Discussion== | ==Discussion== | ||
* | *ADA has a polyhistidine tag, which is a motif of at least 5 histidine residues. The column is nickel because proteins including ADA with polyhistidine tags have high affinities for it. Imidazole has the same functional groups as the histidine residues and they both show absorbancy peaks at 280nm. The machine was baselined so that imidazole's peaks would interfere with ADA's. | ||
*The spectra show that most ADA was collected in the second tube for each sample. | *The spectra show that most ADA was collected in the second tube for each sample. | ||
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Objectives
Procedure
DataThese absorbance peaks show the amount of ADA in each tube. Discussion
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