User:Michael F. Nagle/Notebook/Chem 571/2012/10/02: Difference between revisions
From OpenWetWare
No edit summary |
|||
Line 9: | Line 9: | ||
#Prepare reagents for HRP assay and complete HRP assay with and without gold nanoparticles to see how they affect the reaction. | #Prepare reagents for HRP assay and complete HRP assay with and without gold nanoparticles to see how they affect the reaction. | ||
==Procedure== | ==Procedure== | ||
#HRP procedure [[3760-Protocol HRP AbsorbanceAssay.pdf]] was followed | |||
#1mL horseradish peroxidase was dissolved in 1mL distilled water. | #1mL horseradish peroxidase was dissolved in 1mL distilled water. | ||
#A .2M buffer of sodium phosphate was made at a pH of 7. | #A .2M buffer of sodium phosphate was made at a pH of 7. | ||
#.0017M hydrogen peroxide solution was made by adding 1mL 30% hydrogen peroxide to 100mL distilled water. 1mL of hydrogen peroxide solution was diluted to 50mL with 49mL of sodium phosphate buffer for 34μM solution. | #.0017M hydrogen peroxide solution was made by adding 1mL 30% hydrogen peroxide to 100mL distilled water. 1mL of hydrogen peroxide solution was diluted to 50mL with 49mL of sodium phosphate buffer for 34μM solution. | ||
#698μM iodophenol solution was made in 1mL DMSO, since it's not soluble in water. 2.5mM 4-aminoantipyrine solution was prepared with the sodium phosphate buffer. | #698μM iodophenol solution was made in 1mL DMSO, since it's not soluble in water. 2.5mM 4-aminoantipyrine solution was prepared with the sodium phosphate buffer. | ||
==Discussion== | ==Discussion== | ||
#The HRP assays were not completed today, but will be [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/02|10/02]], with the reagents prepared today | #The HRP assays were not completed today, but will be [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/02|10/02]], with the reagents prepared today |
Revision as of 23:10, 4 October 2012
Project name | <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page <html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html> </html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html> |
Objective
Procedure
Discussion
|