User:Michael F. Nagle/Notebook/Chem 571/2012/10/02: Difference between revisions
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==Procedure== | ==Procedure== | ||
#HRP procedure | #HRP procedure<sup>1</sup> was followed. Time was plotted against absorbance on the UV/Vis spectroscoper. | ||
#1mL horseradish peroxidase was dissolved in 1mL distilled water. | #1mL horseradish peroxidase was dissolved in 1mL distilled water. | ||
#A .2M buffer of sodium phosphate was made at a pH of 7. | #A .2M buffer of sodium phosphate was made at a pH of 7. | ||
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#The HRP assays were not completed today, but will be [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/02|10/02]], with the reagents prepared today | #The HRP assays were not completed today, but will be [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/02|10/02]], with the reagents prepared today | ||
==References== | |||
1. Horseradish Peroxidase Assay. <i>Gold Biotechnology</i> [[Image:3760-Protocol HRP AbsorbanceAssay.pdf]] | |||
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Revision as of 22:01, 6 December 2012
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Objective
Procedure
Discussion
References1. Horseradish Peroxidase Assay. Gold Biotechnology File:3760-Protocol HRP AbsorbanceAssay.pdf |