Objective
- Prepare reagents for HRP assay and complete HRP assay with and without gold nanoparticles to see how they affect the reaction.
Procedure
- HRP procedure File:3760-Protocol HRP AbsorbanceAssay.pdf was followed
- 1mL horseradish peroxidase was dissolved in 1mL distilled water.
- A .2M buffer of sodium phosphate was made at a pH of 7.
- .0017M hydrogen peroxide solution was made by adding 1mL 30% hydrogen peroxide to 100mL distilled water. 1mL of hydrogen peroxide solution was diluted to 50mL with 49mL of sodium phosphate buffer for 34μM solution.
- 698μM iodophenol solution was made in 1mL DMSO, since it's not soluble in water. 2.5mM 4-aminoantipyrine solution was prepared with the sodium phosphate buffer.
- Abigail E. Miller 11:46, 7 October 2012 (EDT):what is different? you did not follow it exactly.
Discussion
- The HRP assays were not completed today, but will be 10/02, with the reagents prepared today
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