User:Michael F. Nagle/Notebook/Chem 571/2012/10/03: Difference between revisions
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**Varying concentrations of gold nanoparticles will be added. | **Varying concentrations of gold nanoparticles will be added. | ||
**The HRP concentration was increased from 1.5μM to 2.3μM because it did not produce a peak at first. | **The HRP concentration was increased from 1.5μM to 2.3μM because it did not produce a peak at first. | ||
*For six cuvettes to have 1.7mM hydrogen peroxide, 18μM iodophenol, 156.25μM AAP and 1.5μM and 2.3μM HRP, the following volumes of the respective stock solutions (made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/02|10/02]]) were added: | |||
**750μL 30% hydrogen peroxide solution | |||
**10μL .17M iodophenol solution | |||
**35μL 2.5mM AAP solution | |||
**15 μL 23μM HRP solution first, which did not produce a peak | |||
***The rest were done with 22μL 23μM HRP solution<br><BR> | |||
{| | {| | ||
|+ Amount of gold and water used in each cuvette | |+ Amount of gold and water used in each cuvette | ||
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|100||530 | |100||530 | ||
|}<br> | |}<br> | ||
**The amount of stock solution needed of each reagant was calculated as such: | |||
**M<sub>1</sub>V<sub>1</sub>=M<sub>2</sub>V<sub>2</sub> | |||
**.023mM HRP stock * .001L = .0023mM HRP * volume needed | |||
** 22μL HRP stock needed | |||
==Data== | ==Data== |
Revision as of 22:43, 6 December 2012
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Objective
Procedure
Data
Discussion
ReferencesNames Removed. Horseradish Peroxidase Assays. File:HRP Assays.pdf |