User:Michael F. Nagle/Notebook/Chem 571/2012/10/16: Difference between revisions
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==Objectives== | |||
*Mutate ADA via PCR mutation | |||
**Mutated ADA is intended for nucleation of AuNPs | |||
*Produce chemiluminescence by adding luminol to HRP assay | |||
==PCR Mutation== | ==PCR Mutation== | ||
* The procedure for [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation]] was followed | * The procedure for [[AU Biomaterials Design Lab:Protocols/PCR Mutation Protocol|PCR Mutation]] was followed | ||
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**Solutions were stored in the freezer. | **Solutions were stored in the freezer. | ||
==HRP Chemiluniscence Assay== | ==HRP Chemiluniscence Assay== | ||
*[[User:Mary_Mendoza/Notebook/ | *[[User:Mary_Mendoza/Notebook/CHEM_571_Experimental_Biological_Chemistry_I/2012/10/16]] prepared 30mM luminol stock. Iodophenol, HRP and H<sub>2</sub>O<sub>2</sub> stock prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/02|10/2]] were used. | ||
*The reaction solutions prepared contained .18mM iodophenol, .1mM H2O2 and 3mM luminol in 1mL quartz cuvettes. | *The reaction solutions prepared contained .18mM iodophenol, .1mM H2O2 and 3mM luminol in 1mL quartz cuvettes. | ||
*2mM HRP added to the solution did not produce luminescence, nor did 10mM. | *2mM HRP added to the solution did not produce luminescence, nor did 10mM. |
Revision as of 00:45, 7 December 2012
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Objectives
PCR Mutation
HRP Chemiluniscence Assay
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