User:Michael F. Nagle/Notebook/Chem 571/2012/10/23

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(Autocreate 2012/10/23 Entry for User:Michael_F._Nagle/Notebook/Chem_571)
Current revision (05:29, 7 December 2012) (view source)
(Discussion)
 
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==Entry title==
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==Objectives==
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* Insert content here...
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*Express ADA with <i>E. Coli</i>
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**ADA [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/28|will be used to nucleate AuNPs]]
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*Analyze 60-170 [Au/BSA] samples prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|yesterday]] via atomic absorbance spectroscopy to determine concentration of AuNPs in solution
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==Starter and Expression Culturess==
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* The [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|Expression Culture]] and [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|Starter Culture]] procedures were followed with the same modifications as on [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/18|9/18]]. These cultures are to be used to grow more <i>E. Coli</i>, this time with a plasmid coding for mutated Adenosine Deaminase (ADA)
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* Samples of Au/BSA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week]] were analyzed by Atomic Absorbtion Spectroscopy.
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**Before they were analyzed, solutions were centrifuged at 3000 rpm at 10°C for 5 min and fluid was separated from fibers. This is because the fibers would clog the instrument's tubing.
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**Between samples, water was run in order to clear residue.
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==Data==
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[[Image:AAgold2.jpg]]
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==Discussion==
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*Our AA spectra is very similar to the one obtained [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/04|9/4]] for 60-170 [Au/BSA]. Both show a drop to near-zero at 128, after an increase. This time the value at 100 is near-zero, which defies the trend and the previous results and may possibly be due to experimental error.
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Objectives

  • Express ADA with E. Coli
  • Analyze 60-170 [Au/BSA] samples prepared yesterday via atomic absorbance spectroscopy to determine concentration of AuNPs in solution

Starter and Expression Culturess

  • The Expression Culture and Starter Culture procedures were followed with the same modifications as on 9/18. These cultures are to be used to grow more E. Coli, this time with a plasmid coding for mutated Adenosine Deaminase (ADA)
  • Samples of Au/BSA made last week were analyzed by Atomic Absorbtion Spectroscopy.
    • Before they were analyzed, solutions were centrifuged at 3000 rpm at 10°C for 5 min and fluid was separated from fibers. This is because the fibers would clog the instrument's tubing.
    • Between samples, water was run in order to clear residue.

Data

Image:AAgold2.jpg

Discussion

  • Our AA spectra is very similar to the one obtained 9/4 for 60-170 [Au/BSA]. Both show a drop to near-zero at 128, after an increase. This time the value at 100 is near-zero, which defies the trend and the previous results and may possibly be due to experimental error.


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