User:Michael F. Nagle/Notebook/Chem 571/2012/10/23: Difference between revisions
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==Objectives== | |||
*Express ADA with <i>E. Coli</i> | |||
**ADA [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/28|will be used to nucleate AuNPs]] | |||
*Analyze 60-170 [Au/BSA] samples prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|yesterday]] via atomic absorbance spectroscopy to determine concentration of AuNPs in solution | |||
==Starter and Expression Culturess== | ==Starter and Expression Culturess== | ||
* The [[AU_Biomaterials_Design_Lab:Protocols/Expression_Culture_Media|Expression Culture]] and [[AU_Biomaterials_Design_Lab:Protocols/Starter_Culture_Media|Starter Culture]] procedures were followed with the same modifications as on [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/18|9/18]]. These cultures are to be used to grow more <i>E. Coli</i>, this time with a plasmid coding for mutated Adenosine Deaminase (ADA) | |||
* Samples of Au/BSA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|last week]] were analyzed by Atomic Absorbtion Spectroscopy. | |||
**Before they were analyzed, solutions were centrifuged at 3000 rpm at 10°C for 5 min and fluid was separated from fibers. This is because the fibers would clog the instrument's tubing. | |||
**Between samples, water was run in order to clear residue. | |||
==Data== | ==Data== | ||
[[Image:AAgold2.jpg]] | |||
==Discussion== | |||
*Our AA spectra is very similar to the one obtained [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/04|9/4]] for 60-170 [Au/BSA]. Both show a drop to near-zero at 128, after an increase. This time the value at 100 is near-zero, which defies the trend and the previous results and may possibly be due to experimental error. | |||
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Latest revision as of 22:10, 26 September 2017
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Objectives
Starter and Expression Culturess
DataDiscussion
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