User:Michael F. Nagle/Notebook/Chem 571/2012/10/24: Difference between revisions
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==Objectives== | |||
*Continue expression of ADA | |||
*Transform mutated plasmid to <i>E. Coli</i> | |||
*Analyze 60-170 [Au/BSA] and [Au/Lysozyme] via UV/Vis | |||
==ADA expression== | ==ADA expression== | ||
* I came in several hours earlier than usual, at 9am, to resuspend cells in fresh LB. | * I came in several hours earlier than usual, at 9am, to resuspend cells in fresh LB. | ||
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[[Image:Bsauvlast.png]] | [[Image:Bsauvlast.png]] | ||
[[Image:Lyzfirst.png]] | [[Image:Lyzfirst.png]] | ||
==Discussion== | |||
*The results were similar to those of the [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/04|set analyzed 9/4]]. A drop to near-zero is seen at the mole ratio 130 [Au/BSA] | |||
*The [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/11|set before this]] showed highest abs. at 130, and had a gradual drop until near-zero at 134. | |||
*Since these data sets do not corroborate eachother, more trials are needed. So far only our second set corroborates data from Bakshi, et. al<sup>1</sup> | |||
=References== | |||
#Bakshi, M.S.; Kaur, H.; Khullar, P.; Banipal, T. S.l; Kaur, G.; Singh, N. Protein Films of Bovine Serum Albumen Conjugated Gold Nanoparticles: A Synthetic Route from Bioconjugated Nanoparticles to Biodegradable Protein Films.<i> J. Phys. Chem.</i> C, 2011, 115 (7), pp 2982–2992 | |||
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Revision as of 03:23, 7 December 2012
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Objectives
ADA expression
Transformation of Mutated Plasmid
UV/Vis of Au and BSA/Lysozyme
DataDiscussion
References=
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