User:Michael F. Nagle/Notebook/Chem 571/2012/10/24

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(Autocreate 2012/10/24 Entry for User:Michael_F._Nagle/Notebook/Chem_571)
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==Entry title==
==Entry title==
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# 5μL of the DNA solution prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/16|last week]] was added to 40μL cells
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# The solution was placed on a heat block for 30 seconds and put on ice.
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# The solution was incubated at 37<sup>o</sup>C and 225rpm for 1 hour
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## 250μL Lysogeny Broth (LB) was added
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# The solution was incubated again with the same specifications
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# Solutions with Lysozyme and BSA prepared [[[[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/16|last week]]|last week]] were analyzed via UV/Vis. There was no peak at 520nm, so it was determined that heating at 50°C was insufficient. The solutions went back in the oven for 4 hours at 80°C, which is the same temperature BSA was seen succesfully unfolding at.
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# Solutions with Au and BSA prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/16|last week]] were analyzed via UV/Vis.
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Revision as of 14:12, 24 October 2012

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  1. 5μL of the DNA solution prepared last week was added to 40μL cells
  2. The solution was placed on a heat block for 30 seconds and put on ice.
  3. The solution was incubated at 37oC and 225rpm for 1 hour
    1. 250μL Lysogeny Broth (LB) was added
  4. The solution was incubated again with the same specifications
  1. Solutions with Lysozyme and BSA prepared [[last week|last week]] were analyzed via UV/Vis. There was no peak at 520nm, so it was determined that heating at 50°C was insufficient. The solutions went back in the oven for 4 hours at 80°C, which is the same temperature BSA was seen succesfully unfolding at.
  2. Solutions with Au and BSA prepared last week were analyzed via UV/Vis.


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