User:Michael F. Nagle/Notebook/Chem 571/2012/11/06: Difference between revisions
From OpenWetWare
(Autocreate 2012/11/06 Entry for User:Michael_F._Nagle/Notebook/Chem_571) |
(fix raw html notebook nav) |
||
(4 intermediate revisions by one other user not shown) | |||
Line 2: | Line 2: | ||
|- | |- | ||
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | |style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span> | ||
|style="background-color: #F2F2F2" align="center"| | |style="background-color: #F2F2F2" align="center"|[[File:Report.png|frameless|link={{#sub:{{FULLPAGENAME}}|0|-11}}]][[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|[[File:Resultset_previous.png|frameless|link={{#lnpreventry:{{FULLPAGENAME}}}}]][[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]] }}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]][[File:Resultset_next.png|frameless|link={{#lnnextentry:{{FULLPAGENAME}}}}]]}} | ||
|- | |- | ||
| colspan="2"| | | colspan="2"| | ||
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | ||
== | ==Objectives== | ||
* | *Transform Adenosine Deaminase in cells | ||
*Purify protein via Fast Protein Liquid Chromatography (FPLC) | |||
==Procedure== | |||
*The prodedure for [[AU_Biomaterials_Design_Lab:Protocols/Transformation_Protocol|transformation]] was followed, just like on [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/24|10/24]] but with the following modifications | |||
**20μL DNA solution was added to 30μL solution with E. Coli cells. | |||
**250μL Lysogeny Broth (LB) was used instead of SOC medium. | |||
**Single 200μL plates were prepared. No 50μL plates were made. | |||
**Plates were incubated at 37<sup>o</sup>C | |||
*Adenosine Deaminase prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] was purified via FPLC [[User:Michael_F._Nagle/Notebook/Chem_571/2012/09/26|as was done 9/26]] | |||
** Pump flow was 5mL/minute | |||
Latest revision as of 22:13, 26 September 2017
Project name | Main project page Previous entry Next entry |
Objectives
Procedure
|