User:Michael F. Nagle/Notebook/Chem 571/2012/11/06

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Objectives

  • Transform Adenosine Deaminase in cells
  • Purify protein via Fast Protein Liquid Chromatography (FPLC)

Procedure

  • The prodedure for [AU_Biomaterials_Design_Lab:Protocols/PCR_Mutation_Protocol|PCR Mutation] was followed, with the following modifications
    • 20μL DNA solution was added to 30μL solution with E. Coli cells.
    • 250μL Lysogeny Broth (LB) was used instead of SOC medium.
    • Single 200μL plates were prepared. No 50μL plates were made.
  • Adenosine Deaminase prepared [User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23] was purified via FPLC
    • Pump flow was 5mL/minute



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