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 Project name
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Objectives
- Transform Adenosine Deaminase in cells
- Purify protein via Fast Protein Liquid Chromatography (FPLC)
Procedure
- The prodedure for PCR Mutation was followed, with the following modifications
- 20μL DNA solution was added to 30μL solution with E. Coli cells.
- 250μL Lysogeny Broth (LB) was used instead of SOC medium.
- Single 200μL plates were prepared. No 50μL plates were made.
- Adenosine Deaminase prepared Oct. 23 was purified via FPLC
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Project name
