User:Michael F. Nagle/Notebook/Chem 571/2012/11/06

From OpenWetWare

Jump to: navigation, search
Project name Main project page
Previous entry      Next entry


  • Transform Adenosine Deaminase in cells
  • Purify protein via Fast Protein Liquid Chromatography (FPLC)


  • The prodedure for transformation was followed, just like on 10/24 but with the following modifications
    • 20μL DNA solution was added to 30μL solution with E. Coli cells.
    • 250μL Lysogeny Broth (LB) was used instead of SOC medium.
    • Single 200μL plates were prepared. No 50μL plates were made.
    • Plates were incubated at 37oC
  • Adenosine Deaminase prepared Oct. 23 was purified via FPLC [User:Michael_F._Nagle/Notebook/Chem_571/2012/09/26|as was done 9/26]]
    • Pump flow was 5mL/minute

Personal tools