- Transform Adenosine Deaminase in cells
- Purify protein via Fast Protein Liquid Chromatography (FPLC)
- The prodedure for transformation was followed, just like on 10/24 but with the following modifications
- 20μL DNA solution was added to 30μL solution with E. Coli cells.
- 250μL Lysogeny Broth (LB) was used instead of SOC medium.
- Single 200μL plates were prepared. No 50μL plates were made.
- Plates were incubated at 37oC
- Adenosine Deaminase prepared Oct. 23 was purified via FPLC as was done 9/26