User:Michael F. Nagle/Notebook/Chem 571/2012/11/07

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(Bradford Assay)
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A standard curve was obtained with the following concentrations of BSA:
A standard curve was obtained with the following concentrations of BSA:
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[table]
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{| {{table}}
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[tr]
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| align="center" style="background:#f0f0f0;"|'''volume BSA (uL)'''
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[th]volume BSA (uL)[/th]
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| align="center" style="background:#f0f0f0;"|'''volume H2O (uL'''
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[th]volume H2O (uL)[/th]
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| align="center" style="background:#f0f0f0;"|'''[BSA] (mg/mL)'''
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[th]concentration BSA (mg/mL)[/th]
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| align="center" style="background:#f0f0f0;"|'''[BSA] (M)'''
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[th]concentration BSA (molarity)[/th]
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|-
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[/tr]
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| 0||30||0||0.00E+00
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[tr]
+
|-
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[td]0[/td]
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| 5||25||0.24||3.61E-06
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[td]30[/td]
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|-
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[td]0[/td]
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| 10||20||0.15||2.26E-06
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[td]0.00E+00[/td]
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|-
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[/tr]
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| 15||15||0.13||2.01E-06
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[tr]
+
|-
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[td]5[/td]
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| 20||10||0.15||2.26E-06
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[td]25[/td]
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|-
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[td].24[/td]
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| 25||5||0.24||3.61E-06
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[td]3.61E-06[/td]
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|-
-
[/tr]
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| 30||0||1||1.51E-05
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[tr]
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|-
-
[td]10[/td]
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|
-
[td]20[/td]
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|}
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[td].15[/td]
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[td]2.26E-06[/td]
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-
[/tr]
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-
[tr]
+
-
[td]15[/td]
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-
[td]15[/td]
+
-
[td].13[/td]
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-
[td]2.01E-06[/td]
+
-
[/tr]
+
-
[tr]
+
-
[td]20[/td]
+
-
[td]10[/td]
+
-
[td].15[/td]
+
-
[td]2.26E-06[/td]
+
-
[/tr]
+
-
[tr]
+
-
[td]25[/td]
+
-
[td]5[/td]
+
-
[td].24[/td]
+
-
[td]3.61E-06[/td]
+
-
[/tr]
+
-
[tr]
+
-
[td]30[/td]
+
-
[td]0[/td]
+
-
[td]1[/td]
+
-
[td]1.51E-05[/td]
+
-
[/tr]
+
-
[/table]
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<Br>
<Br>
*The ADA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] was analyzed.
*The ADA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] was analyzed.

Revision as of 13:51, 7 November 2012

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Bradford Assay

A standard curve was obtained with the following concentrations of BSA:

volume BSA (uL) volume H2O (uL [BSA] (mg/mL) [BSA] (M)
03000.00E+00
5250.243.61E-06
10200.152.26E-06
15150.132.01E-06
20100.152.26E-06
2550.243.61E-06
30011.51E-05


  • The ADA made Oct. 23 was analyzed.
  • The ADA's peak was compared to those of BSA's linear regression in order to determine concentration.


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