User:Michael F. Nagle/Notebook/Chem 571/2012/11/07

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(Bradford Assay)
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==Objective==
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*Determine concentration of ADA prepared [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] via Bradford Assay
==Bradford Assay==
==Bradford Assay==
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*The ADA made [[User:Michael_F._Nagle/Notebook/Chem_571/2012/10/23|Oct. 23]] was analyzed.
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*The ADA was analyzed via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration.
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*The ADA's peak was compared to those of BSA's linear regression in order to determine concentration.
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Revision as of 13:53, 7 November 2012

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Objective

  • Determine concentration of ADA prepared Oct. 23 via Bradford Assay

Bradford Assay

A standard curve was obtained with the following concentrations of BSA:

volume BSA (uL) volume H2O (uL [BSA] (mg/mL) [BSA] (M)
03000.00E+00
5250.243.61E-06
10200.152.26E-06
15150.132.01E-06
20100.152.26E-06
2550.243.61E-06
30011.51E-05


  • The ADA was analyzed via UV/Vis and it's peak was compared to the linear regression of BSA's in order to determine concentration.


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