User:Michael F. Nagle/Notebook/Chem 571/2012/11/07

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Bradford Assay

A standard curve was obtained with the following concentrations of BSA:

volume BSA (uL) volume H2O (uL [BSA] (mg/mL) [BSA] (M)
0 30 0 0.00E+00
5 25 0.24 3.61E-06
10 20 0.15 2.26E-06
15 15 0.13 2.01E-06
20 10 0.15 2.26E-06
25 5 0.24 3.61E-06
30 0 1 1.51E-05


  • The ADA made Oct. 23 was analyzed.
  • The ADA's peak was compared to those of BSA's linear regression in order to determine concentration.


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