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Analysis of Au/ADA samples
- Samples prepared last week were analyzed via UV/Vis
Discussion
- Peaks are seen at 430nm but not 528nm, the point of interest for AuNPs. The peaks at 420nm do not change relative to the change in background. Change in background has in the past been attributed to the use of plastic, rather than quartz cuvettes.
- It's possible that the salt in solution is competing with the HAuCl4 and preventing it's reduction. Dialyzed ADA should be analyzed.
- As can be seen in the graph below, samples of pre-dialysis ADA analyzed by the other group did not have the peak at 430nm. This indicates that the peak was not caused by the imidazole, tris or NaCl present, and may have been caused by some other contamination or experimental error.
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