User:Michael F. Nagle/Notebook/Chem 571/2012/11/27: Difference between revisions
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==Objectives== | |||
*Complete ADA activity assay | |||
*Prepare solutions with post-dialysis ADA and Au at same mole ratios that were analyzed [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/20|11/20]] | |||
==Procedure== | ==Procedure== | ||
*The dialysis tubing was openned and the contents of both tubes was added to seperate falcon tubes via pipetman. One of these samples, Frac #8, which is [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/07|54.4μM]] was used to prepare solutions of 25-200 [Au/ADA] | *The dialysis tubing was openned and the contents of both tubes was added to seperate falcon tubes via pipetman. One of these samples, Frac #8, which is [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/07|54.4μM]] was used to prepare solutions of 25-200 [Au/ADA] | ||
**Solutions were prepared with 1.5μM ADA and the following mole ratios of [Au/ADA]: 25, 50, 75, 100, 125, 150, 175 and 200 | **Solutions were prepared with 1.5μM ADA and the following mole ratios of [Au/ADA]: 25, 50, 75, 100, 125, 150, 175 and 200 | ||
**Solutions were capped with foil and heated at 85<sup>o</sup>C for 4 hours. | **Solutions were capped with foil and heated at 85<sup>o</sup>C for 4 hours. | ||
**[[User:Mary_Mendoza/Notebook/ | **[[User:Mary_Mendoza/Notebook/CHEM_571_Experimental_Biological_Chemistry_I/2012/11/27|Mary Mendoza]] completed the ADA activity assay but the spectra aren't on Dropbox so I will have the data on here once I hear back about it's location | ||
Revision as of 05:59, 7 December 2012
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Objectives
Procedure
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