User:Michael F. Nagle/Notebook/Chem 571/2012/11/27

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==Objectives==
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*Complete ADA activity assay
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*Prepare solutions with post-dialysis ADA and Au at same mole ratios that were analyzed [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/20|11/20]]
==Procedure==
==Procedure==
*The dialysis tubing was openned and the contents of both tubes was added to seperate falcon tubes via pipetman. One of these samples, Frac #8, which is [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/07|54.4μM]] was used to prepare solutions of 25-200 [Au/ADA]
*The dialysis tubing was openned and the contents of both tubes was added to seperate falcon tubes via pipetman. One of these samples, Frac #8, which is [[User:Michael_F._Nagle/Notebook/Chem_571/2012/11/07|54.4μM]] was used to prepare solutions of 25-200 [Au/ADA]
**Solutions were prepared with 1.5μM ADA and the following mole ratios of [Au/ADA]: 25, 50, 75, 100, 125, 150, 175 and 200
**Solutions were prepared with 1.5μM ADA and the following mole ratios of [Au/ADA]: 25, 50, 75, 100, 125, 150, 175 and 200
**Solutions were capped with foil and heated at 85<sup>o</sup>C for 4 hours.
**Solutions were capped with foil and heated at 85<sup>o</sup>C for 4 hours.
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**[[User:Mary_Mendoza/Notebook/Chem_571:_Gold_Nanoparticles/2012/11/27|Mary Mendoza]] completed the ADA activity assay but the spectra aren't on Dropbox so I will have the data on here once I hear back about it's location
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**[[User:Mary_Mendoza/Notebook/CHEM_571_Experimental_Biological_Chemistry_I/2012/11/27|Mary Mendoza]] completed the ADA activity assay but the spectra aren't online so I won't have the data on here until I hear back about it's location

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Objectives

  • Complete ADA activity assay
  • Prepare solutions with post-dialysis ADA and Au at same mole ratios that were analyzed 11/20

Procedure

  • The dialysis tubing was openned and the contents of both tubes was added to seperate falcon tubes via pipetman. One of these samples, Frac #8, which is 54.4μM was used to prepare solutions of 25-200 [Au/ADA]
    • Solutions were prepared with 1.5μM ADA and the following mole ratios of [Au/ADA]: 25, 50, 75, 100, 125, 150, 175 and 200
    • Solutions were capped with foil and heated at 85oC for 4 hours.
    • Mary Mendoza completed the ADA activity assay but the spectra aren't online so I won't have the data on here until I hear back about it's location



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