User:Michael F. Nagle/Notebook/Chem 571/2013/01/30

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==Objective==
 
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*Prepare stock solutions of adenosine and phosphate buffer
 
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*Prepare aliquots of ADA stock solution
 
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**Stocks will be used for ADA activity assay next week
 
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==Preparation of adenosine stock solution==
 
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*10mL of 15mM solution of adenosine was prepared
 
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** .015M = x/.01
 
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**0.0015mol adenosine * 267.24mol/g adenosine = 401mg adenosine
 
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**401mg adenosine was weighed and added to 10mL phosphate buffer.
 
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*The 15mM solution was diluted to 5mM
 
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**.01L * .015M = .005M * x
 
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**0.03L total volume for 5mM
 
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**0.02L buffer was added to bring the solution to 30mL and 5mM
 
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==Preparation of phosphate buffer==
 
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*3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.
 
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*pH was brought to 7.4 NaOH
 
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==Preparation of ADA aliquots==
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*[[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics|Dhea Patel]] prepared 68mL 0.5unit/mL ADA stock solution
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*This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.
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