User:Michael F. Nagle/Notebook/Chem 571/2013/01/30

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(Preparation of phosphate buffer)
(Objective)
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==Objective==
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*Prepare stock solutions of adenosine and phosphate buffer
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*Prepare aliquots of ADA stock solution
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**Stocks will be used for ADA activity assay next week
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==Preparation of adenosine stock solution==
==Preparation of adenosine stock solution==

Revision as of 17:22, 8 May 2013

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Preparation of adenosine stock solution

  • 10mL of 15mM solution of adenosine was prepared
    • .015M = x/.01
    • 0.0015mol adenosine * 267.24mol/g adenosine = 401mg adenosine
    • 401mg adenosine was weighed and added to 10mL phosphate buffer.
  • The 15mM solution was diluted to 5mM
    • .01L * .015M = .005M * x
    • 0.03L total volume for 5mM
    • 0.02L buffer was added to bring the solution to 30mL and 5mM

Preparation of phosphate buffer

  • 3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.
  • pH was brought to 7.4 using NaOH

Preparation of ADA aliquots

  • Dhea Patel measured out 1.7g 0.5unit/mL ADA
  • Stock solution was prepared by adding 68 mL phosphate buffer to the ADA
  • This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.




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