User:Michael F. Nagle/Notebook/Chem 571/2013/01/30

From OpenWetWare

< User:Michael F. Nagle | Notebook | Chem 571 | 2013 | 01(Difference between revisions)
Jump to: navigation, search
Current revision (16:22, 8 May 2013) (view source)
(Preparation of ADA aliquots)
 
(5 intermediate revisions not shown.)
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-
==Objective==
 
-
*Prepare stock solutions of adenosine and phosphate buffer
 
-
*Prepare aliquots of ADA stock solution
 
-
**Stocks will be used for ADA activity assay next week
 
-
==Preparation of adenosine stock solution==
 
-
*10mL of 15mM solution of adenosine was prepared
 
-
** .015M = x/.01
 
-
**0.0015mol adenosine * 267.24mol/g adenosine = 401mg adenosine
 
-
**401mg adenosine was weighed and added to 10mL phosphate buffer.
 
-
*The 15mM solution was diluted to 5mM
 
-
**.01L * .015M = .005M * x
 
-
**0.03L total volume for 5mM
 
-
**0.02L buffer was added to bring the solution to 30mL and 5mM
 
-
==Preparation of phosphate buffer==
 
-
*3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.
 
-
*pH was brought to 7.4 NaOH
 
-
==Preparation of ADA aliquots==
+
 
-
*[[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics|Dhea Patel]] prepared 68mL 0.5unit/mL ADA stock solution
+
 
-
*This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.
+

Current revision

Project name Main project page
Previous entry      Next entry






Personal tools