User:Michael F. Nagle/Notebook/Chem 571/2013/01/30

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(Preparation of adenosine stock solution)
Current revision (16:22, 8 May 2013) (view source)
(Preparation of ADA aliquots)
 
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==Preparation of phosphate buffer==
 
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*3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.
 
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*pH was brought to 7.4 using NaOH
 
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==Preparation of ADA aliquots==
 
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*[[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics|Dhea Patel]] measured out 1.7g 0.5unit/mL ADA
 
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*Stock solution was prepared by adding 68 mL phosphate buffer to the ADA
 
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*This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.
 

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