User:Michael F. Nagle/Notebook/Chem 571/2013/01/30

(Difference between revisions)

Revision as of 15:55, 30 January 2013

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10mL of 15mM solution of adenosine was prepared
- .015M = x/.01
- 0.0015mol adenosine * 267.24mol/g adenosine = 401mg adenosine
- 401mg adenosine was weighed and added to 10mL phosphate buffer.
The 15mM solution was diluted to 5mM
- .01L * .015M = .005M * x
- 0.03L total volume for 5mM
- 0.02L buffer was added to bring the solution to 30mL and 5mM

Dhea Patel prepared 68mL 0.5unit/mL ADA stock solution
This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.

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-==Entry title==
+==Preparation of adenosine stock solution==
-* Insert content here...
+*10mL of 15mM solution of adenosine was prepared
+** .015M = x/.01
+**0.0015mol adenosine * 267.24mol/g adenosine = 401mg adenosine
+**401mg adenosine was weighed and added to 10mL phosphate buffer.
+*The 15mM solution was diluted to 5mM
+**.01L * .015M = .005M * x
+**0.03L total volume for 5mM
+**0.02L buffer was added to bring the solution to 30mL and 5mM
+==Preparation of phosphate buffer==
+*3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.
+*pH was brought to 7.4 NaOH
+==Preparation of ADA aliquots==
+*[[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics|Dhea Patel]] prepared 68mL 0.5unit/mL ADA stock solution
+*This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.