User:Michael F. Nagle/Notebook/Chem 571/2013/01/30: Difference between revisions
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== | ==Preparation of adenosine stock solution== | ||
* | *10mL of 15mM solution of adenosine was prepared | ||
** .015M = x/.01 | |||
**0.0015mol adenosine * 267.24mol/g adenosine = 401mg adenosine | |||
**401mg adenosine was weighed and added to 10mL phosphate buffer. | |||
*The 15mM solution was diluted to 5mM | |||
**.01L * .015M = .005M * x | |||
**0.03L total volume for 5mM | |||
**0.02L buffer was added to bring the solution to 30mL and 5mM | |||
==Preparation of phosphate buffer== | |||
*3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken. | |||
*pH was brought to 7.4 NaOH | |||
==Preparation of ADA aliquots== | |||
*[[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics|Dhea Patel]] prepared 68mL 0.5unit/mL ADA stock solution | |||
*This was divided into 68 1mL centrifuge tubes, which were stored in the freezer. | |||
Revision as of 12:55, 30 January 2013
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Preparation of adenosine stock solution
Preparation of phosphate buffer
Preparation of ADA aliquots
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