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Objective
- Prepare stock solutions of adenosine and phosphate buffer
- Prepare aliquots of ADA stock solution
- Stocks will be used for ADA activity assay next week
Preparation of adenosine stock solution
- 10mL of 15mM solution of adenosine was prepared
- .015M = x/.01
- 0.0015mol adenosine * 267.24mol/g adenosine = 401mg adenosine
- 401mg adenosine was weighed and added to 10mL phosphate buffer.
- The 15mM solution was diluted to 5mM
- .01L * .015M = .005M * x
- 0.03L total volume for 5mM
- 0.02L buffer was added to bring the solution to 30mL and 5mM
Preparation of phosphate buffer
- 3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.
- pH was brought to 7.4 NaOH
Preparation of ADA aliquots
- Dhea Patel measured out 1.7g 0.5unit/mL ADA
- Stock solution was prepared by adding 68 mL phosphate buffer to the ADA
- This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.
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