User:Michael F. Nagle/Notebook/Chem 571/2013/01/30 - Revision history

Mary Mendoza: /* Preparation of ADA aliquots */

Mary Mendoza — Wed, 08 May 2013 21:22:49 GMT

Preparation of ADA aliquots

←Older revision		Revision as of 21:22, 8 May 2013
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-
-	~~==Preparation of ADA aliquots==~~
-	*[[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics\|Dhea Patel]] measured out 1.7g 0.5unit/mL ADA
-	*Stock solution was prepared by adding 68 mL phosphate buffer to the ADA
-	*This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.

Mary Mendoza: /* Preparation of phosphate buffer */

Mary Mendoza — Wed, 08 May 2013 21:22:36 GMT

Preparation of phosphate buffer

←Older revision		Revision as of 21:22, 8 May 2013
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-	~~==Preparation of phosphate buffer==~~	+
-	*3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.	+
-	*pH was brought to 7.4 using NaOH	+

	==Preparation of ADA aliquots==		==Preparation of ADA aliquots==

Mary Mendoza: /* Preparation of adenosine stock solution */

Mary Mendoza — Wed, 08 May 2013 21:22:24 GMT

Preparation of adenosine stock solution

←Older revision		Revision as of 21:22, 8 May 2013
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-	~~==Preparation of adenosine stock solution==~~	+
-	*10mL of 15mM solution of adenosine was prepared	+
-	** .015M = x/.01	+
-	*0.0015mol adenosine 267.24mol/g adenosine = 401mg adenosine	+
-	**401mg adenosine was weighed and added to 10mL phosphate buffer.	+
-	*The 15mM solution was diluted to 5mM	+
-	*.01L .015M = .005M * x	+
-	**0.03L total volume for 5mM	+
-	**0.02L buffer was added to bring the solution to 30mL and 5mM	+

	==Preparation of phosphate buffer==		==Preparation of phosphate buffer==

Mary Mendoza: /* Objective */

Mary Mendoza — Wed, 08 May 2013 21:22:16 GMT

Objective

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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	~~==Objective==~~	+
-	*Prepare stock solutions of adenosine and phosphate buffer	+
-	*Prepare aliquots of ADA stock solution	+
-	**Stocks will be used for ADA activity assay next week	+

	==Preparation of adenosine stock solution==		==Preparation of adenosine stock solution==

Michael F. Nagle: /* Preparation of phosphate buffer */

Michael F. Nagle — Wed, 30 Jan 2013 19:58:17 GMT

Preparation of phosphate buffer

←Older revision		Revision as of 19:58, 30 January 2013
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	==Preparation of phosphate buffer==		==Preparation of phosphate buffer==
	*3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.		*3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.
-	*pH was brought to 7.4 NaOH	+	*pH was brought to 7.4 using NaOH

	==Preparation of ADA aliquots==		==Preparation of ADA aliquots==

Michael F. Nagle: /* Preparation of ADA aliquots */

Michael F. Nagle — Wed, 30 Jan 2013 19:57:52 GMT

Preparation of ADA aliquots

←Older revision		Revision as of 19:57, 30 January 2013
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	==Preparation of ADA aliquots==		==Preparation of ADA aliquots==
-	*[[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics\|Dhea Patel]] ~~prepared 68mL~~ 0.5unit/mL ADA ~~stock~~ solution	+	*[[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics\|Dhea Patel]] measured out 1.7g 0.5unit/mL ADA
		+	*Stock solution was prepared by adding 68 mL phosphate buffer to the ADA
	*This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.		*This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.

Michael F. Nagle at 19:56, 30 January 2013

Michael F. Nagle — Wed, 30 Jan 2013 19:56:35 GMT

←Older revision		Revision as of 19:56, 30 January 2013
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	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
		+	==Objective==
		+	*Prepare stock solutions of adenosine and phosphate buffer
		+	*Prepare aliquots of ADA stock solution
		+	**Stocks will be used for ADA activity assay next week
		+
	==Preparation of adenosine stock solution==		==Preparation of adenosine stock solution==
	*10mL of 15mM solution of adenosine was prepared		*10mL of 15mM solution of adenosine was prepared

Michael F. Nagle at 19:55, 30 January 2013

Michael F. Nagle — Wed, 30 Jan 2013 19:55:06 GMT

←Older revision		Revision as of 19:55, 30 January 2013
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	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Preparation of adenosine stock solution==
-	* ~~Insert content here~~...	+	*10mL of 15mM solution of adenosine was prepared
		+	** .015M = x/.01
		+	*0.0015mol adenosine 267.24mol/g adenosine = 401mg adenosine
		+	**401mg adenosine was weighed and added to 10mL phosphate buffer.
		+	*The 15mM solution was diluted to 5mM
		+	*.01L .015M = .005M * x
		+	**0.03L total volume for 5mM
		+	**0.02L buffer was added to bring the solution to 30mL and 5mM
		+
		+	==Preparation of phosphate buffer==
		+	*3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.
		+	*pH was brought to 7.4 NaOH
		+
		+	==Preparation of ADA aliquots==
		+	*[[User:Dhea_Patel/Notebook/CHEM_572:_ADA%26Inhibitor_Kinetics\|Dhea Patel]] prepared 68mL 0.5unit/mL ADA stock solution
		+	*This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.
		+
		+

Michael F. Nagle: Autocreate 2013/01/30 Entry for User:Michael_F._Nagle/Notebook/Chem_571

Michael F. Nagle — Wed, 30 Jan 2013 19:43:17 GMT

Autocreate 2013/01/30 Entry for User:Michael_F._Nagle/Notebook/Chem_571

New page

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|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
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==Entry title==
* Insert content here...


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