User:Michael F. Nagle/Notebook/Chem 571/2013/01/30

From OpenWetWare
Jump to navigationJump to search
Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

  • Prepare stock solutions of adenosine and phosphate buffer
  • Prepare aliquots of ADA stock solution
    • Stocks will be used for ADA activity assay next week

Preparation of adenosine stock solution

  • 10mL of 15mM solution of adenosine was prepared
    • .015M = x/.01
    • 0.0015mol adenosine * 267.24mol/g adenosine = 401mg adenosine
    • 401mg adenosine was weighed and added to 10mL phosphate buffer.
  • The 15mM solution was diluted to 5mM
    • .01L * .015M = .005M * x
    • 0.03L total volume for 5mM
    • 0.02L buffer was added to bring the solution to 30mL and 5mM

Preparation of phosphate buffer

  • 3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.
  • pH was brought to 7.4 NaOH

Preparation of ADA aliquots

  • Dhea Patel measured out 1.7g 0.5unit/mL ADA
  • Stock solution was prepared by adding 68 mL phosphate buffer to the ADA
  • This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.




Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Michael_F._Nagle/Notebook/Chem_571/2013/01/30&oldid=671687"