User:Michael F. Nagle/Notebook/Chem 571/2013/01/30

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Preparation of phosphate buffer

  • 3.72g NaH2PO4 and 13.08 Na2HPO4 were added to 1.2L water, which was shaken.
  • pH was brought to 7.4 using NaOH

Preparation of ADA aliquots

  • Dhea Patel measured out 1.7g 0.5unit/mL ADA
  • Stock solution was prepared by adding 68 mL phosphate buffer to the ADA
  • This was divided into 68 1mL centrifuge tubes, which were stored in the freezer.




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