< User:Michael F. Nagle | Notebook | Chem 571 | 2013 | 02(Difference between revisions)
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| | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> | | <!-- ##### DO NOT edit above this line unless you know what you are doing. ##### --> |
| - | ==Objective==
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| - | Repeat ADA Assay 2 times for additional data
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| | | | |
| - | ==Description==
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| - | *Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
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| - | *5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
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| - | **Dilution Tables are located below
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| | | | |
| - | ==Concentration Tables==
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| - | This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
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| | | | |
| - | {| {{table}}
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| - | | align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
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| - | | align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
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| - | | align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
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| - | | align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
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| - | | align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
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| - | |-
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| - | | 5000||200||800||1000||1
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| - | |-
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| - | | 5000||150||850||750||1
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| - | |-
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| - | | 5000||100||900||500||1
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| - | |-
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| - | | 5000||40||960||200||1
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| - | |-
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| - | | 5000||16||984||80||1
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| - | |-
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| - | | 5000||6.4||993.6||32||1
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| - | |}
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| | | | |
| - | This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
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| | | | |
| - | {| {{table}}
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| - | | align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
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| - | | align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
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| - | | align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
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| - | | align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)'''
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| - | | align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
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| - | | align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
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| - | | align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
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| - | | align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
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| - | |-
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| - | | 0.5||0.02||0.00333||1000||0.6||200||2.38||3
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| - | |-
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| - | | 0.5||0.02||0.00333||750||0.6||150||2.38||3
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| - | |-
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| - | | 0.5||0.02||0.00333||500||0.6||100||2.38||3
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| - | |-
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| - | | 0.5||0.02||0.00333||200||0.6||40||2.38||3
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| - | |-
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| - | | 0.5||0.02||0.00333||80||0.6||16||2.38||3
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| - | |-
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| - | | 0.5||0.02||0.00333||32||0.6||6.4||2.38||3
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| - | |}
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| - |
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| - | ==Data==
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| - | Run#1
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| - |
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| - | {| {{table}}
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| - | | align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
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| - | | align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
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| - | |-
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| - | | 200||0.000116022
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| - | |-
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| - | | 150||0.000192818
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| - | |-
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| - | | 100||0.000139227
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| - | |-
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| - | | 40||5.55556E-06
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| - | |-
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| - | | 16||6.68508E-05
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| - | |-
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| - | | 6.4||0.000039
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| - | |}
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| - |
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| - | {| {{table}}
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| - | | align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
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| - | | align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
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| - | |-
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| - | | 0.005||8619.047619
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| - | |-
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| - | | 0.006666667||5186.246418
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| - | |-
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| - | | 0.01||7182.539683
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| - | |-
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| - | | 0.025||180000
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| - | |-
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| - | | 0.0625||14958.67769
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| - | |-
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| - | | 0.15625||25641.02564
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| - | |}
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| - |
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| - |
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| - | Run#2
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| - |
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| - | {| {{table}}
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| - | | align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
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| - | | align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
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| - | |-
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| - | | 200||0.00021989
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| - | |-
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| - | | 150||0.000147514
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| - | |-
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| - | | 100||0.000144751
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| - | |-
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| - | | 40||0.00010884
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| - | |-
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| - | | 16||8.12155E-05
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| - | |-
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| - | | 6.4||3.8674E-05
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| - | |}
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| - |
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| - | {| {{table}}
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| - | | align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
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| - | | align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
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| - | |-
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| - | | 0.005||4547.738693
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| - | |-
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| - | | 0.006666667||6779.026217
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| - | |-
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| - | | 0.01||6908.396947
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| - | |-
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| - | | 0.025||9187.817259
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| - | |-
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| - | | 0.0625||12312.92517
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| - | |-
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| - | | 0.15625||25857.14286
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| - | |}
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