User:Michael F. Nagle/Notebook/Chem 571/2013/02/12: Difference between revisions

From OpenWetWare
Jump to navigationJump to search
(Autocreate 2013/02/12 Entry for User:Michael_F._Nagle/Notebook/Chem_571)
 
No edit summary
Line 6: Line 6:
| colspan="2"|
| colspan="2"|
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
==Entry title==
==Objective==
* Insert content here...
Repeat ADA Assay 2 times for additional data
 
==Description==
*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
**Dilution Tables are located below
 
==Concentration Tables==
This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
|-
| 5000||200||800||1000||1
|-
| 5000||150||850||750||1
|-
| 5000||100||900||500||1
|-
| 5000||40||960||200||1
|-
| 5000||16||984||80||1
|-
| 5000||6.4||993.6||32||1
|}
 
This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
|-
| 0.5||0.02||0.00333||1000||0.6||200||2.38||3
|-
| 0.5||0.02||0.00333||750||0.6||150||2.38||3
|-
| 0.5||0.02||0.00333||500||0.6||100||2.38||3
|-
| 0.5||0.02||0.00333||200||0.6||40||2.38||3
|-
| 0.5||0.02||0.00333||80||0.6||16||2.38||3
|-
| 0.5||0.02||0.00333||32||0.6||6.4||2.38||3
|}
 
==Data==
Run#1
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
|-
| 200||0.000116022
|-
| 150||0.000192818
|-
| 100||0.000139227
|-
| 40||5.55556E-06
|-
| 16||6.68508E-05
|-
| 6.4||0.000039
|}
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
|-
| 0.005||8619.047619
|-
| 0.006666667||5186.246418
|-
| 0.01||7182.539683
|-
| 0.025||180000
|-
| 0.0625||14958.67769
|-
| 0.15625||25641.02564
|}
 
 
Run#2
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''Concentration of Adenosine (uM)'''
| align="center" style="background:#f0f0f0;"|'''Average Velocity over 180s (nm/sec)'''
|-
| 200||0.00021989
|-
| 150||0.000147514
|-
| 100||0.000144751
|-
| 40||0.00010884
|-
| 16||8.12155E-05
|-
| 6.4||3.8674E-05
|}
 
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''1/Concentration of Adenosine (uM)'''
| align="center" style="background:#f0f0f0;"|'''1/Velocity (nm/sec)'''
|-
| 0.005||4547.738693
|-
| 0.006666667||6779.026217
|-
| 0.01||6908.396947
|-
| 0.025||9187.817259
|-
| 0.0625||12312.92517
|-
| 0.15625||25857.14286
|}




Revision as of 18:27, 21 February 2013

Project name <html><img src="/images/9/94/Report.png" border="0" /></html> Main project page
<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>Previous entry<html>&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;&nbsp;</html>Next entry<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>

Objective

Repeat ADA Assay 2 times for additional data

Description

  • Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
  • 5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
    • Dilution Tables are located below

Concentration Tables

This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.

[Old Adenosine Stock Solution] (uM) Volume Adenosine Stock Solution added (uL) Volume Adenosine Phosphate Buffer added (uL) [New Adenosine Stock Solution] (uL) Volume New Adenosine Stock Solution (mL)
5000 200 800 1000 1
5000 150 850 750 1
5000 100 900 500 1
5000 40 960 200 1
5000 16 984 80 1
5000 6.4 993.6 32 1

This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.

[ADA Stock Solution] (U/mL) Volume ADA Stock Solution Added (mL) Final [ADA] (U/mL) [Adenosine Stock Solution] (mL) Volume Adenosine Stock Solution Added (mL) Final [Adenosine] (uM) Volume 0.05 M Phosphate Buffer Added (mL) Total Volume (mL)
0.5 0.02 0.00333 1000 0.6 200 2.38 3
0.5 0.02 0.00333 750 0.6 150 2.38 3
0.5 0.02 0.00333 500 0.6 100 2.38 3
0.5 0.02 0.00333 200 0.6 40 2.38 3
0.5 0.02 0.00333 80 0.6 16 2.38 3
0.5 0.02 0.00333 32 0.6 6.4 2.38 3

Data

Run#1

Concentration of Adenosine (uM) Average Velocity over 180s (nm/sec)
200 0.000116022
150 0.000192818
100 0.000139227
40 5.55556E-06
16 6.68508E-05
6.4 0.000039
1/Concentration of Adenosine (uM) 1/Velocity (nm/sec)
0.005 8619.047619
0.006666667 5186.246418
0.01 7182.539683
0.025 180000
0.0625 14958.67769
0.15625 25641.02564


Run#2

Concentration of Adenosine (uM) Average Velocity over 180s (nm/sec)
200 0.00021989
150 0.000147514
100 0.000144751
40 0.00010884
16 8.12155E-05
6.4 3.8674E-05
1/Concentration of Adenosine (uM) 1/Velocity (nm/sec)
0.005 4547.738693
0.006666667 6779.026217
0.01 6908.396947
0.025 9187.817259
0.0625 12312.92517
0.15625 25857.14286



Retrieved from "https://openwetware.org/mediawiki/index.php?title=User:Michael_F._Nagle/Notebook/Chem_571/2013/02/12&oldid=678423"