User:Michael F. Nagle/Notebook/Chem 571/2013/02/12: Difference between revisions

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==Concentration Tables==
This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.


{| {{table}}
| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
|-
| 5000||200||800||1000||1
|-
| 5000||150||850||750||1
|-
| 5000||100||900||500||1
|-
| 5000||40||960||200||1
|-
| 5000||16||984||80||1
|-
| 5000||6.4||993.6||32||1
|}
This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
{| {{table}}
| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)'''
| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
|-
| 0.5||0.02||0.00333||1000||0.6||200||2.38||3
|-
| 0.5||0.02||0.00333||750||0.6||150||2.38||3
|-
| 0.5||0.02||0.00333||500||0.6||100||2.38||3
|-
| 0.5||0.02||0.00333||200||0.6||40||2.38||3
|-
| 0.5||0.02||0.00333||80||0.6||16||2.38||3
|-
| 0.5||0.02||0.00333||32||0.6||6.4||2.38||3
|}


==Data==
==Data==

Revision as of 14:26, 8 May 2013

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Data

Run#1

Concentration of Adenosine (uM) Average Velocity over 180s (nm/sec)
200 0.000116022
150 0.000192818
100 0.000139227
40 5.55556E-06
16 6.68508E-05
6.4 0.000039
1/Concentration of Adenosine (uM) 1/Velocity (nm/sec)
0.005 8619.047619
0.006666667 5186.246418
0.01 7182.539683
0.025 180000
0.0625 14958.67769
0.15625 25641.02564


Run#2

Concentration of Adenosine (uM) Average Velocity over 180s (nm/sec)
200 0.00021989
150 0.000147514
100 0.000144751
40 0.00010884
16 8.12155E-05
6.4 3.8674E-05
1/Concentration of Adenosine (uM) 1/Velocity (nm/sec)
0.005 4547.738693
0.006666667 6779.026217
0.01 6908.396947
0.025 9187.817259
0.0625 12312.92517
0.15625 25857.14286



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