User:Michael F. Nagle/Notebook/Chem 571/2013/02/12

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(Concentration Tables)
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==Concentration Tables==
 
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This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''[Old Adenosine Stock Solution] (uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution added (uL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Phosphate Buffer added (uL)'''
 
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| align="center" style="background:#f0f0f0;"|'''[New Adenosine Stock Solution] (uL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume New Adenosine Stock Solution (mL)'''
 
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|-
 
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| 5000||200||800||1000||1
 
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|-
 
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| 5000||150||850||750||1
 
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|-
 
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| 5000||100||900||500||1
 
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|-
 
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| 5000||40||960||200||1
 
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|-
 
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| 5000||16||984||80||1
 
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|-
 
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| 5000||6.4||993.6||32||1
 
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|}
 
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This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
 
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{| {{table}}
 
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| align="center" style="background:#f0f0f0;"|'''[ADA Stock Solution] (U/mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume ADA Stock Solution Added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Final [ADA] (U/mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''[Adenosine Stock Solution] (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume Adenosine Stock Solution Added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Final [Adenosine] (uM)'''
 
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| align="center" style="background:#f0f0f0;"|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
 
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| align="center" style="background:#f0f0f0;"|'''Total Volume (mL)'''
 
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|-
 
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| 0.5||0.02||0.00333||1000||0.6||200||2.38||3
 
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|-
 
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| 0.5||0.02||0.00333||750||0.6||150||2.38||3
 
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|-
 
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| 0.5||0.02||0.00333||500||0.6||100||2.38||3
 
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|-
 
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| 0.5||0.02||0.00333||200||0.6||40||2.38||3
 
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|-
 
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| 0.5||0.02||0.00333||80||0.6||16||2.38||3
 
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|-
 
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| 0.5||0.02||0.00333||32||0.6||6.4||2.38||3
 
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|}
 
==Data==
==Data==

Revision as of 16:26, 8 May 2013

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Data

Run#1

Concentration of Adenosine (uM) Average Velocity over 180s (nm/sec)
2000.000116022
1500.000192818
1000.000139227
405.55556E-06
166.68508E-05
6.40.000039
1/Concentration of Adenosine (uM) 1/Velocity (nm/sec)
0.0058619.047619
0.0066666675186.246418
0.017182.539683
0.025180000
0.062514958.67769
0.1562525641.02564


Run#2

Concentration of Adenosine (uM) Average Velocity over 180s (nm/sec)
2000.00021989
1500.000147514
1000.000144751
400.00010884
168.12155E-05
6.43.8674E-05
1/Concentration of Adenosine (uM) 1/Velocity (nm/sec)
0.0054547.738693
0.0066666676779.026217
0.016908.396947
0.0259187.817259
0.062512312.92517
0.1562525857.14286



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