User:Michael F. Nagle/Notebook/Chem 571/2013/02/12 - Revision history

Mary Mendoza: /* Data */

Mary Mendoza — Wed, 08 May 2013 21:26:19 GMT

Data

←Older revision		Revision as of 21:26, 8 May 2013
Line 11:		Line 11:


-
-	~~==Data==~~
-	~~Run#1~~
-
-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Concentration of Adenosine (uM)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Average Velocity over 180s (nm/sec)'''~~
-	\|-
-	~~\| 200\|\|0.000116022~~
-	\|-
-	~~\| 150\|\|0.000192818~~
-	\|-
-	~~\| 100\|\|0.000139227~~
-	\|-
-	~~\| 40\|\|5.55556E-06~~
-	\|-
-	~~\| 16\|\|6.68508E-05~~
-	\|-
-	~~\| 6.4\|\|0.000039~~
-	\|}
-
-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''1/Concentration of Adenosine (uM)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''1/Velocity (nm/sec)'''~~
-	\|-
-	~~\| 0.005\|\|8619.047619~~
-	\|-
-	~~\| 0.006666667\|\|5186.246418~~
-	\|-
-	~~\| 0.01\|\|7182.539683~~
-	\|-
-	~~\| 0.025\|\|180000~~
-	\|-
-	~~\| 0.0625\|\|14958.67769~~
-	\|-
-	~~\| 0.15625\|\|25641.02564~~
-	\|}
-
-
-	~~Run#2~~
-
-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Concentration of Adenosine (uM)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Average Velocity over 180s (nm/sec)'''~~
-	\|-
-	~~\| 200\|\|0.00021989~~
-	\|-
-	~~\| 150\|\|0.000147514~~
-	\|-
-	~~\| 100\|\|0.000144751~~
-	\|-
-	~~\| 40\|\|0.00010884~~
-	\|-
-	~~\| 16\|\|8.12155E-05~~
-	\|-
-	~~\| 6.4\|\|3.8674E-05~~
-	\|}
-
-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''1/Concentration of Adenosine (uM)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''1/Velocity (nm/sec)'''~~
-	\|-
-	~~\| 0.005\|\|4547.738693~~
-	\|-
-	~~\| 0.006666667\|\|6779.026217~~
-	\|-
-	~~\| 0.01\|\|6908.396947~~
-	\|-
-	~~\| 0.025\|\|9187.817259~~
-	\|-
-	~~\| 0.0625\|\|12312.92517~~
-	\|-
-	~~\| 0.15625\|\|25857.14286~~
-	\|}

Mary Mendoza: /* Concentration Tables */

Mary Mendoza — Wed, 08 May 2013 21:26:05 GMT

Concentration Tables

←Older revision		Revision as of 21:26, 8 May 2013
Line 10:		Line 10:


-	~~==Concentration Tables==~~
-	~~This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.~~

-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''[Old Adenosine Stock Solution] (uM)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Volume Adenosine Stock Solution added (uL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Volume Adenosine Phosphate Buffer added (uL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''[New Adenosine Stock Solution] (uL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Volume New Adenosine Stock Solution (mL)'''~~
-	\|-
-	~~\| 5000\|\|200\|\|800\|\|1000\|\|1~~
-	\|-
-	~~\| 5000\|\|150\|\|850\|\|750\|\|1~~
-	\|-
-	~~\| 5000\|\|100\|\|900\|\|500\|\|1~~
-	\|-
-	~~\| 5000\|\|40\|\|960\|\|200\|\|1~~
-	\|-
-	~~\| 5000\|\|16\|\|984\|\|80\|\|1~~
-	\|-
-	~~\| 5000\|\|6.4\|\|993.6\|\|32\|\|1~~
-	\|}
-
-	~~This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.~~
-
-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''[ADA Stock Solution] (U/mL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Volume ADA Stock Solution Added (mL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Final [ADA] (U/mL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''[Adenosine Stock Solution] (mL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Volume Adenosine Stock Solution Added (mL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Final [Adenosine] (uM)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Volume 0.05 M Phosphate Buffer Added (mL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Total Volume (mL)'''~~
-	\|-
-	~~\| 0.5\|\|0.02\|\|0.00333\|\|1000\|\|0.6\|\|200\|\|2.38\|\|3~~
-	\|-
-	~~\| 0.5\|\|0.02\|\|0.00333\|\|750\|\|0.6\|\|150\|\|2.38\|\|3~~
-	\|-
-	~~\| 0.5\|\|0.02\|\|0.00333\|\|500\|\|0.6\|\|100\|\|2.38\|\|3~~
-	\|-
-	~~\| 0.5\|\|0.02\|\|0.00333\|\|200\|\|0.6\|\|40\|\|2.38\|\|3~~
-	\|-
-	~~\| 0.5\|\|0.02\|\|0.00333\|\|80\|\|0.6\|\|16\|\|2.38\|\|3~~
-	\|-
-	~~\| 0.5\|\|0.02\|\|0.00333\|\|32\|\|0.6\|\|6.4\|\|2.38\|\|3~~
-	\|}

	==Data==		==Data==

Mary Mendoza: /* Description */

Mary Mendoza — Wed, 08 May 2013 21:25:53 GMT

Description

←Older revision		Revision as of 21:25, 8 May 2013
Line 8:		Line 8:


-	~~==Description==~~	+
-	*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer	+
-	*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:	+
-	**Dilution Tables are located below	+

	==Concentration Tables==		==Concentration Tables==

Mary Mendoza: /* Objective */

Mary Mendoza — Wed, 08 May 2013 21:25:29 GMT

Objective

←Older revision		Revision as of 21:25, 8 May 2013
Line 6:		Line 6:
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	~~==Objective==~~	+
-	~~Repeat ADA Assay 2 times for additional data~~	+

	==Description==		==Description==

Michael F. Nagle at 01:27, 22 February 2013

Michael F. Nagle — Fri, 22 Feb 2013 01:27:09 GMT

←Older revision		Revision as of 01:27, 22 February 2013
Line 6:		Line 6:
	\| colspan="2"\|		\| colspan="2"\|
	<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->		<!-- ##### DO NOT edit above this line unless you know what you are doing. ##### -->
-	==~~Entry title~~==	+	==Objective==
-	* ~~Insert content here~~...	+	Repeat ADA Assay 2 times for additional data
		+
		+	==Description==
		+	*Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
		+	*5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
		+	**Dilution Tables are located below
		+
		+	==Concentration Tables==
		+	This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
		+
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''[Old Adenosine Stock Solution] (uM)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Volume Adenosine Stock Solution added (uL)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Volume Adenosine Phosphate Buffer added (uL)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''[New Adenosine Stock Solution] (uL)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Volume New Adenosine Stock Solution (mL)'''
		+	\|-
		+	\| 5000\|\|200\|\|800\|\|1000\|\|1
		+	\|-
		+	\| 5000\|\|150\|\|850\|\|750\|\|1
		+	\|-
		+	\| 5000\|\|100\|\|900\|\|500\|\|1
		+	\|-
		+	\| 5000\|\|40\|\|960\|\|200\|\|1
		+	\|-
		+	\| 5000\|\|16\|\|984\|\|80\|\|1
		+	\|-
		+	\| 5000\|\|6.4\|\|993.6\|\|32\|\|1
		+	\|}
		+
		+	This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
		+
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''[ADA Stock Solution] (U/mL)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Volume ADA Stock Solution Added (mL)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Final [ADA] (U/mL)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''[Adenosine Stock Solution] (mL)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Volume Adenosine Stock Solution Added (mL)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Final [Adenosine] (uM)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Volume 0.05 M Phosphate Buffer Added (mL)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Total Volume (mL)'''
		+	\|-
		+	\| 0.5\|\|0.02\|\|0.00333\|\|1000\|\|0.6\|\|200\|\|2.38\|\|3
		+	\|-
		+	\| 0.5\|\|0.02\|\|0.00333\|\|750\|\|0.6\|\|150\|\|2.38\|\|3
		+	\|-
		+	\| 0.5\|\|0.02\|\|0.00333\|\|500\|\|0.6\|\|100\|\|2.38\|\|3
		+	\|-
		+	\| 0.5\|\|0.02\|\|0.00333\|\|200\|\|0.6\|\|40\|\|2.38\|\|3
		+	\|-
		+	\| 0.5\|\|0.02\|\|0.00333\|\|80\|\|0.6\|\|16\|\|2.38\|\|3
		+	\|-
		+	\| 0.5\|\|0.02\|\|0.00333\|\|32\|\|0.6\|\|6.4\|\|2.38\|\|3
		+	\|}
		+
		+	==Data==
		+	Run#1
		+
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''Concentration of Adenosine (uM)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Average Velocity over 180s (nm/sec)'''
		+	\|-
		+	\| 200\|\|0.000116022
		+	\|-
		+	\| 150\|\|0.000192818
		+	\|-
		+	\| 100\|\|0.000139227
		+	\|-
		+	\| 40\|\|5.55556E-06
		+	\|-
		+	\| 16\|\|6.68508E-05
		+	\|-
		+	\| 6.4\|\|0.000039
		+	\|}
		+
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''1/Concentration of Adenosine (uM)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''1/Velocity (nm/sec)'''
		+	\|-
		+	\| 0.005\|\|8619.047619
		+	\|-
		+	\| 0.006666667\|\|5186.246418
		+	\|-
		+	\| 0.01\|\|7182.539683
		+	\|-
		+	\| 0.025\|\|180000
		+	\|-
		+	\| 0.0625\|\|14958.67769
		+	\|-
		+	\| 0.15625\|\|25641.02564
		+	\|}
		+
		+
		+	Run#2
		+
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''Concentration of Adenosine (uM)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''Average Velocity over 180s (nm/sec)'''
		+	\|-
		+	\| 200\|\|0.00021989
		+	\|-
		+	\| 150\|\|0.000147514
		+	\|-
		+	\| 100\|\|0.000144751
		+	\|-
		+	\| 40\|\|0.00010884
		+	\|-
		+	\| 16\|\|8.12155E-05
		+	\|-
		+	\| 6.4\|\|3.8674E-05
		+	\|}
		+
		+	{\| {{table}}
		+	\| align="center" style="background:#f0f0f0;"\|'''1/Concentration of Adenosine (uM)'''
		+	\| align="center" style="background:#f0f0f0;"\|'''1/Velocity (nm/sec)'''
		+	\|-
		+	\| 0.005\|\|4547.738693
		+	\|-
		+	\| 0.006666667\|\|6779.026217
		+	\|-
		+	\| 0.01\|\|6908.396947
		+	\|-
		+	\| 0.025\|\|9187.817259
		+	\|-
		+	\| 0.0625\|\|12312.92517
		+	\|-
		+	\| 0.15625\|\|25857.14286
		+	\|}

Michael F. Nagle: Autocreate 2013/02/12 Entry for User:Michael_F._Nagle/Notebook/Chem_571

Michael F. Nagle — Fri, 22 Feb 2013 01:27:01 GMT

Autocreate 2013/02/12 Entry for User:Michael_F._Nagle/Notebook/Chem_571

New page

{|{{table}} width="800"
|-
|style="background-color: #EEE"|[[Image:owwnotebook_icon.png|128px]]<span style="font-size:22px;"> Project name</span>
|style="background-color: #F2F2F2" align="center"|<html><img src="/images/9/94/Report.png" border="0" /></html> [[{{#sub:{{FULLPAGENAME}}|0|-11}}|Main project page]]<br />{{#if:{{#lnpreventry:{{FULLPAGENAME}}}}|<html><img src="/images/c/c3/Resultset_previous.png" border="0" /></html>[[{{#lnpreventry:{{FULLPAGENAME}}}}{{!}}Previous entry]]<html>      </html>}}{{#if:{{#lnnextentry:{{FULLPAGENAME}}}}|[[{{#lnnextentry:{{FULLPAGENAME}}}}{{!}}Next entry]]<html><img src="/images/5/5c/Resultset_next.png" border="0" /></html>}}
|-
| colspan="2"|

==Entry title==
* Insert content here...


|}

__NOTOC__

←Older revision		Revision as of 21:26, 8 May 2013
Line 11:		Line 11:


-
-	~~==Data==~~
-	~~Run#1~~
-
-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Concentration of Adenosine (uM)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Average Velocity over 180s (nm/sec)'''~~
-	\|-
-	~~\| 200\|\|0.000116022~~
-	\|-
-	~~\| 150\|\|0.000192818~~
-	\|-
-	~~\| 100\|\|0.000139227~~
-	\|-
-	~~\| 40\|\|5.55556E-06~~
-	\|-
-	~~\| 16\|\|6.68508E-05~~
-	\|-
-	~~\| 6.4\|\|0.000039~~
-	\|}
-
-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''1/Concentration of Adenosine (uM)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''1/Velocity (nm/sec)'''~~
-	\|-
-	~~\| 0.005\|\|8619.047619~~
-	\|-
-	~~\| 0.006666667\|\|5186.246418~~
-	\|-
-	~~\| 0.01\|\|7182.539683~~
-	\|-
-	~~\| 0.025\|\|180000~~
-	\|-
-	~~\| 0.0625\|\|14958.67769~~
-	\|-
-	~~\| 0.15625\|\|25641.02564~~
-	\|}
-
-
-	~~Run#2~~
-
-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Concentration of Adenosine (uM)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Average Velocity over 180s (nm/sec)'''~~
-	\|-
-	~~\| 200\|\|0.00021989~~
-	\|-
-	~~\| 150\|\|0.000147514~~
-	\|-
-	~~\| 100\|\|0.000144751~~
-	\|-
-	~~\| 40\|\|0.00010884~~
-	\|-
-	~~\| 16\|\|8.12155E-05~~
-	\|-
-	~~\| 6.4\|\|3.8674E-05~~
-	\|}
-
-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''1/Concentration of Adenosine (uM)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''1/Velocity (nm/sec)'''~~
-	\|-
-	~~\| 0.005\|\|4547.738693~~
-	\|-
-	~~\| 0.006666667\|\|6779.026217~~
-	\|-
-	~~\| 0.01\|\|6908.396947~~
-	\|-
-	~~\| 0.025\|\|9187.817259~~
-	\|-
-	~~\| 0.0625\|\|12312.92517~~
-	\|-
-	~~\| 0.15625\|\|25857.14286~~
-	\|}

←Older revision		Revision as of 21:26, 8 May 2013
Line 10:		Line 10:


-	~~==Concentration Tables==~~
-	~~This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.~~

-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''[Old Adenosine Stock Solution] (uM)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Volume Adenosine Stock Solution added (uL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Volume Adenosine Phosphate Buffer added (uL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''[New Adenosine Stock Solution] (uL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Volume New Adenosine Stock Solution (mL)'''~~
-	\|-
-	~~\| 5000\|\|200\|\|800\|\|1000\|\|1~~
-	\|-
-	~~\| 5000\|\|150\|\|850\|\|750\|\|1~~
-	\|-
-	~~\| 5000\|\|100\|\|900\|\|500\|\|1~~
-	\|-
-	~~\| 5000\|\|40\|\|960\|\|200\|\|1~~
-	\|-
-	~~\| 5000\|\|16\|\|984\|\|80\|\|1~~
-	\|-
-	~~\| 5000\|\|6.4\|\|993.6\|\|32\|\|1~~
-	\|}
-
-	~~This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.~~
-
-	~~{\| {{table}}~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''[ADA Stock Solution] (U/mL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Volume ADA Stock Solution Added (mL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Final [ADA] (U/mL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''[Adenosine Stock Solution] (mL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Volume Adenosine Stock Solution Added (mL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Final [Adenosine] (uM)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Volume 0.05 M Phosphate Buffer Added (mL)'''~~
-	~~\| align="center" style="background:#f0f0f0;"\|'''Total Volume (mL)'''~~
-	\|-
-	~~\| 0.5\|\|0.02\|\|0.00333\|\|1000\|\|0.6\|\|200\|\|2.38\|\|3~~
-	\|-
-	~~\| 0.5\|\|0.02\|\|0.00333\|\|750\|\|0.6\|\|150\|\|2.38\|\|3~~
-	\|-
-	~~\| 0.5\|\|0.02\|\|0.00333\|\|500\|\|0.6\|\|100\|\|2.38\|\|3~~
-	\|-
-	~~\| 0.5\|\|0.02\|\|0.00333\|\|200\|\|0.6\|\|40\|\|2.38\|\|3~~
-	\|-
-	~~\| 0.5\|\|0.02\|\|0.00333\|\|80\|\|0.6\|\|16\|\|2.38\|\|3~~
-	\|-
-	~~\| 0.5\|\|0.02\|\|0.00333\|\|32\|\|0.6\|\|6.4\|\|2.38\|\|3~~
-	\|}

	==Data==		==Data==