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Project name
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Description
- Using procedure for UV-Vis from 2/6/13 two assays were performed using Adenosine and ADA in phosphate buffer
- 5mM adenosine stock solution was too concentrated and exceeded the capacity of the UV-Vis therefore it was diluted:
- Dilution Tables are located below
Concentration Tables
This table is the volumes of 5mM adenosine and phosphate buffer used to make the new adenosine stock solutions.
[Old Adenosine Stock Solution] (uM)
|
Volume Adenosine Stock Solution added (uL)
|
Volume Adenosine Phosphate Buffer added (uL)
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[New Adenosine Stock Solution] (uL)
|
Volume New Adenosine Stock Solution (mL)
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5000 |
200 |
800 |
1000 |
1
|
5000 |
150 |
850 |
750 |
1
|
5000 |
100 |
900 |
500 |
1
|
5000 |
40 |
960 |
200 |
1
|
5000 |
16 |
984 |
80 |
1
|
5000 |
6.4 |
993.6 |
32 |
1
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This table is the updated table of the volumes and concentrations of solutions that will be added in the cuvette.
[ADA Stock Solution] (U/mL)
|
Volume ADA Stock Solution Added (mL)
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Final [ADA] (U/mL)
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[Adenosine Stock Solution] (mL)
|
Volume Adenosine Stock Solution Added (mL)
|
Final [Adenosine] (uM)
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Volume 0.05 M Phosphate Buffer Added (mL)
|
Total Volume (mL)
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0.5 |
0.02 |
0.00333 |
1000 |
0.6 |
200 |
2.38 |
3
|
0.5 |
0.02 |
0.00333 |
750 |
0.6 |
150 |
2.38 |
3
|
0.5 |
0.02 |
0.00333 |
500 |
0.6 |
100 |
2.38 |
3
|
0.5 |
0.02 |
0.00333 |
200 |
0.6 |
40 |
2.38 |
3
|
0.5 |
0.02 |
0.00333 |
80 |
0.6 |
16 |
2.38 |
3
|
0.5 |
0.02 |
0.00333 |
32 |
0.6 |
6.4 |
2.38 |
3
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Data
Run#1
Concentration of Adenosine (uM)
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Average Velocity over 180s (nm/sec)
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200 |
0.000116022
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150 |
0.000192818
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100 |
0.000139227
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40 |
5.55556E-06
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16 |
6.68508E-05
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6.4 |
0.000039
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1/Concentration of Adenosine (uM)
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1/Velocity (nm/sec)
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0.005 |
8619.047619
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0.006666667 |
5186.246418
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0.01 |
7182.539683
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0.025 |
180000
|
0.0625 |
14958.67769
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0.15625 |
25641.02564
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Run#2
Concentration of Adenosine (uM)
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Average Velocity over 180s (nm/sec)
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200 |
0.00021989
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150 |
0.000147514
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100 |
0.000144751
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40 |
0.00010884
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16 |
8.12155E-05
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6.4 |
3.8674E-05
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1/Concentration of Adenosine (uM)
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1/Velocity (nm/sec)
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0.005 |
4547.738693
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0.006666667 |
6779.026217
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0.01 |
6908.396947
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0.025 |
9187.817259
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0.0625 |
12312.92517
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0.15625 |
25857.14286
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